Amplification of human lymphotropic virus 1 (HTLV-1) DNA segments in patients with tropical spastic paraparesis and asymptomatic seropositive patients
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To amplify proviral genomes from DNA obtained from 13 PET and asymptomatic carriers, a polymerase chain reaction (PCR) was used. A 187 bp segment amplified from the pol gene was observed in 7 (53.8%) samples. A 159 bp tax-rex fragment was visualized in 8 (61.5%) of DNA; only 2 (16.6%) samples amplified the 357 bp 3'LTR. The analysis showed 53.8% of HTVL-1 provirus present in the sample. Two false negatives were detected when comparing ELISA and Immunoblot to PCR results (p=2/13). Criteria for reevaluating ELISA and Immunoblot as diagnostic alternatives were stated.
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