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Since November 1990 and June 1991, three times with three months of intervals, nine different disinfectants in use at the Rafael Uribe Uribe Clinic, ISS-Valle in Calt, Colombia, were studied for their antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa with a new method comprising a combination of minimal inhibitory concentration, mortality curve, and neutralization tests. The results show that the technique in evaluation found inactivity in one disinfectant that by a conventional system (mortaltty curve) was not selected. The new method deserves more evaluations for a routine test in Colombia.

Miryam Astudillo H, Departamento de Microbiología

Departamento de Microbiología, Facul-tad de Salud, Universidad del Valle, Cal!, Colombia.

Alberto Concha E, Sección de Epidemiología

Sección de Epidemiología, Instituto de Seguros Socia-les, Secciona! Valle del Cauca, Cal!, Colombia

Luz E. Daza, Departamento de Microbiología, Facultad de Salud. Universidad del Valle, Cali, Colombia

Departamento de Microbiología, Facultad de Salud. Universidad del Valle, Cali, Colombia

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