@article{Ramírez_Palacios_Gutiérrez_2007, title={Implementation of the isolated vascular tissue model as a device for the validation of medicinal plants: Study of the vasodilator activity of Salvia scutellarioides.}, volume={38}, url={https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/471}, DOI={10.25100/cm.v38i1.471}, abstractNote={<strong>Introduction:</strong> Salvia scutellarioides is a plant with antihypertensive and diuretic properties. In this study, its vasodilator effect is evaluated using the isolated organ technique.<br /> <strong>Objectives:</strong> To standardize the isolated vascular organ, and to study the effect of S. scutellarioides in vasoconstriction by alfa 1 receptors stimulation.<br /> <strong>Methodology:</strong> Rat aortic rings were placed in an organ bath equipment (BIOPAC®), at 37<sup>o</sup> C temperature and constant carbogen gas bubbling. Tension changes were registered with an isometric tension transducer and data acquisition equipment. During the standardization, six curves of concentration-tension with phenylephrine at 1, 3 and 5 hours were registered, in order to determine maximum tension (TM), and the effective concentration 50 (EC50). Finally, three concentration-tension curves were obtained for phenylephrine with S. scutellarioides at a concentration of 10 mg/dl and 20 mg/dl. <br /> <strong>Results:</strong> During the technique standardization, no statistically significant differences were found in TM for phenylephrine at 1, 3 and 5 hours. The administration of S. scutellarioides produced a TM dose dependent reduction with no EC50 changes.<br /> <strong>Discussion:</strong> The implemented isolated organ technique is viable up to 5 hours, and does not produce alfa 1 receptors desensibilization. The TM diminution by S. scutellarioides is explained by a non competitive alfa 1 antagonism effect. Studies to isolate active principles for allowing the initiation of phase I human clinical trials are required.}, number={1}, journal={Colombia Medica}, author={Ramírez, Jorge Hernán and Palacios, Mauricio and Gutiérrez, Oscar}, year={2007}, month={Mar.}, pages={28–39} }