TY - JOUR AU - Moncayo, Jose Ignacio AU - Luligo Espinal, Jenna Samara AU - Santacruz Ibarra, Jorge Javier AU - Álvarez Aldana, Adalucy PY - 2016/03/30 Y2 - 2024/03/29 TI - Relationship between super antigenicity, antimicrobial resistance and origin of Staphylococcus aureus isolated JF - Colombia Medica JA - Colomb Med VL - 47 IS - 1 SE - Original Articles DO - 10.25100/cm.v47i1.1818 UR - https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/1818 SP - 15-20 AB - <div id="__secid242681" class="sec sec-first" style="clear: both; color: #000000; font-family: 'Times New Roman', stixgeneral, serif; font-size: 15.9991px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 21.9988px; orphans: auto; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: #f8f8f8;"><h3 style="font-size: 0.875em; line-height: 1.2857; margin: 1.2856em 0px 0.6428em; color: #724128; font-family: arial, helvetica, clean, sans-serif;">Introduction:</h3><p id="__pid242685" class="p p-first-last" style="margin: 0.6923em 0px;"><em>Staphylococcus aureus</em><span> </span>is a pathogen that causes food poisoning as well as hospital and community acquired infections.</p></div><div id="__secid242691" class="sec" style="clear: both; color: #000000; font-family: 'Times New Roman', stixgeneral, serif; font-size: 15.9991px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 21.9988px; orphans: auto; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: #f8f8f8;"><h3 style="font-size: 0.875em; line-height: 1.2857; margin: 1.2856em 0px 0.6428em; color: #724128; font-family: arial, helvetica, clean, sans-serif;">Objective:</h3><p id="__pid242694" class="p p-first-last" style="margin: 0.6923em 0px;">Establish the profile of superantigen genes among hospital isolates in relation to clinical specimen type, susceptibility to antibiotics and hospital or community acquisition.</p></div><div id="__secid242698" class="sec" style="clear: both; color: #000000; font-family: 'Times New Roman', stixgeneral, serif; font-size: 15.9991px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 21.9988px; orphans: auto; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: #f8f8f8;"><h3 style="font-size: 0.875em; line-height: 1.2857; margin: 1.2856em 0px 0.6428em; color: #724128; font-family: arial, helvetica, clean, sans-serif;">Methods:</h3><p id="__pid251325" class="p p-first-last" style="margin: 0.6923em 0px;">Eighty one isolates obtained from patients at Colombian hospital, were classified by antimicrobial susceptibility, specimen type and hospital or community acquired . The PCR uniplex and multiplex was used for detection of 22 superantigen genes (18 enterotoxins,<span> </span><em>tsst-1</em><span> </span>and three exfoliative toxins).</p></div><div id="__secid251333" class="sec" style="clear: both; color: #000000; font-family: 'Times New Roman', stixgeneral, serif; font-size: 15.9991px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 21.9988px; orphans: auto; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: #f8f8f8;"><h3 style="font-size: 0.875em; line-height: 1.2857; margin: 1.2856em 0px 0.6428em; color: #724128; font-family: arial, helvetica, clean, sans-serif;">Results:</h3><p id="__pid251336" class="p p-first-last" style="margin: 0.6923em 0px;">Ninety five point one percent of isolates harbored one or more of the genes with an average of 5.6 genes. Prevalence of individual genes was variable and the most prevalent was<span> </span><em>seg</em><span> </span>(51.9%). 39 genotypes were obtained, and the genotype<span> </span><em>gimnou</em><span> </span>(complete<span> </span><em>egc<span> </span></em>cluster) was the most prevalent alone (16.0%) and in association with other genes (13.6%). The correlation between presence of superantigens and clinical specimen or antimicrobial susceptibility showed no significant difference. But there was significant difference between presence of superantigens and the origin of the isolates, hospital or community acquired (<em>p</em>= 0.049).</p></div><div id="__secid251357" class="sec sec-last" style="clear: both; color: #000000; font-family: 'Times New Roman', stixgeneral, serif; font-size: 15.9991px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 21.9988px; orphans: auto; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; background-color: #f8f8f8;"><h3 style="font-size: 0.875em; line-height: 1.2857; margin: 1.2856em 0px 0.6428em; color: #724128; font-family: arial, helvetica, clean, sans-serif;">Conclusions:</h3><p id="__pid251360" class="p p-first-last" style="margin: 0.6923em 0px;">The results show the variability of the superantigen genes profile in hospital isolates and shows no conclusive relationship with the clinical sample type and antimicrobial susceptibility, but there was correlation with community and hospital isolates. The analysis of the interplay between virulence, epidemic and antibiotic resistance of bacterial populations is needed to predict the future of infectious diseases.</p></div> ER -