TY - JOUR AU - Cespedes, Nora AU - Valencia, Angela AU - Echeverry, Carlos Alberto AU - Arce-Plata, Maria Isabel AU - Colon, Cristobal AU - Castiñeiras, Daisy E. AU - Hurtado, Paula Margarita AU - Cocho, Jose Angel AU - Herrera, Socrates AU - Arevalo-Herrera, Myriam PY - 2017/09/20 Y2 - 2024/03/28 TI - Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia JF - Colombia Medica JA - Colomb Med VL - 48 IS - 3 SE - Original Articles DO - 10.25100/cm.v48i3.2180 UR - https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/2180 SP - 113-119 AB - <p><strong>Introduction: </strong>Inborn errors of metabolism (IEM) represent an important public health problem due to current diagnosis and treatment limitations, poor life quality of affected patients, and consequent untimely child death. In contrast to classical methods, tandem mass spectrometry (MS/MS) has allowed simultaneous evaluation of multiple metabolites associated with IEM offering higher sensitivity, low false positive rates and high throughput.</p> <p><strong>Aims: </strong>Determine concentration levels for amino acids and acylcarnitines in blood of newborns from Colombia, to establish reference values for further use in diagnosis of IEM.<strong></strong></p> <p><strong>Methods:</strong> Implementation of a method to determine amino acids, acylcarnitines and succinylacetone in newborn dried blood spots using MS/MS, and its application in a cross-sectional study conducted in 891 healthy neonates from Cali and Quibdó cities is described.</p> <p><strong>Results: </strong>fifty seven analytes that allow the diagnosis of 50 different pathologies were tested. The method showed to be linear, precise and accurate. Healthy neonates 2-18 days of age were included, 523 from Cali and 368 from Quibdó; 52% male and 48% female. Age-related differences on the concentration levels of amino acids and acylcarnitines were observed whereas no significant differences by gender were found.</p> <p><strong>Conclusion: </strong>The study has contributed to reveal the usual concentration levels of amino acids, acylcarnitines and succinylacetone that could be used as reference for the establishment of a newborn metabolic screening program in Colombia.</p> ER -