TY - JOUR AU - Cepeda Prado, Efraín AU - López Tobón, Alejandro AU - García Segura, Luis Miguel AU - Cardona Gómez, Gloria Patricia PY - 2008/09/30 Y2 - 2024/03/29 TI - 17 β-estradiol decreases the expression and association of kinases responsible of Tau hyperphosphorylation. JF - Colombia Medica JA - Colomb Med VL - 39 IS - 3 Supl 3 SE - Original Articles DO - 10.25100/cm.v39i3Supl3.604 UR - https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/604 SP - 38-45 AB - <span style="font-weight: bold;"><strong>Introduction:</strong> </span>A predominant molecular component analyzed in the study of neurodegenerative diseases is the presence of the Tau-GSK3<span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span> complex and its association with protein aggregation into the cell. Several evidences show that GSK3<span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span> has an important role in abnormal pattern of the phosphorylation of Tau. However, the molecular events that are governing this complex are unknown. <br /> <span style="font-weight: bold;"><strong>Aim:</strong> </span>To determine the effect of 17 <span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-estradiol treatment on the expression and association of Tau hyperphosphorylation responsible kinases.<br /> <strong><span style="font-weight: bold;">Methods:</span></strong> 17 <span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-estradiol treatments were realized in the hippocampus of ovariectomized adult wistar rats and in hippocampal primary cultures treated with <span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-amiloid. Protein complex association was assessed by co-immunoprecipitation, toxicity assay by LDH release and cell morphologic changes by confocal microscopy. <br /> <strong><span style="font-weight: bold;">Results:</span></strong> Our results show that 17<span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-estradiol produced dissociation of macromolecular complexes like Tau/GSK3<span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>, Tau /GluR2/3, Tau/FAK, and Tau/Fyn in hippocampus of adult rat. In addition the expression of GSK3<span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-ptyr was decreased by the hormonal treatment and this one regulated the defosforilation of Tau in an excitotoxicity model by <span style="font-size: 12pt; font-family: ";Times New Roman";;">β</span>-amiloid. <br /> <strong><span style="font-weight: bold;">Conclusions:</span></strong> It suggests new targets that will contribute to neuroprotection and neuronal plasticity studies mediated by the estrogen. ER -