TY - JOUR AU - Arias, Yazmín Rocío AU - Carrillo, Edward Fabían AU - Aristizabal, Fabio Ancizar PY - 2010/06/24 Y2 - 2024/03/29 TI - Human papillomavirus (HPV) detected in restored plasma DNA from women diagnosed with pre-invasive lesions and invasive cervical cancer JF - Colombia Medica JA - Colomb Med VL - 41 IS - 2 SE - Original Articles DO - UR - https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/696 SP - 148-154 AB - <small><span style="font-family: Arial;"><span style="font-weight: bold;">Objective:</span> To improve the sensitivity of Human Papillomavirus (HPV) detection in plasma from high-grade cervical neoplasia patients (CIN III) and cervical cancer (CC) evaluating any likely correlation with disease stage. </span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Method:</span> We subjected plasma DNA isolates from 112 patients (CIN and ICC) to a pre-PCR restoration treatment to improve detection sensitivity. HPV-specific sequences were detected by conventional PCR both in cervical scrapes and plasma DNA obtained from each patient. For every single DNA sample, both non-restored and restored isolates were PCR analyzed. </span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Results:</span> We detected HPV in plasma DNA isolates with significantly higher efficiency on restored plasma-DNA as compared to each non-restored equivalent, still maintaining close correlation with the clinical stage of the cases. By analyzing plasma-DNA isolates we could classify as HPV positive >50.0% of the cases that were previously known to be positive from the cervical scrape based assay. Interestingly, 100% of the cases in which subtype HPV18 was detected in cervical scrapes were also positive in plasma DNA. </span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Conclusions: </span>Restoration of plasma DNA from cervical cancer patients allows a more sensitive PCR-based HPV detection, maintaining the correlation to disease stage traditionally observed.</span></small> ER -