TY - JOUR AU - Moreno, Luis Hernando AU - Alzate, Alberto PY - 2010/06/24 Y2 - 2024/03/29 TI - Enzyme-linked immunosorbent assay of phenolic glycolipid-I in patients with Hansen’s disease JF - Colombia Medica JA - Colomb Med VL - 41 IS - 2 SE - Original Articles DO - UR - https://colombiamedica.univalle.edu.co/index.php/comedica/article/view/697 SP - 155-160 AB - <small><span style="font-family: Arial;"><span style="font-weight: bold;">Introduction:</span> Hansen’s disease or leprosy is a contagious-infection entity produced by the Hansen bacillus or Mycobacterium leprae. The phenolic glycolipid is a special trisaccharide found in the bacillus cell wall and proved to be specific and immunogenic species during M. leprae.</span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Objective:</span> To determine the presence of the Hansen bacillus enzyme-linked immunoassay (ELISA) method glycolipid phenolic I in a group of patients in Dermatology Consultation at the Valle del Cauca Health Services; these patients were classified as cures or under watch according to criteria established by the World Health Organization (WHO).</span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Methodology:</span> From the data base of the Dermatology Consultation at the Valle del Cauca Health Services, we studied 159 patients with Hansen’s disease who were tested with the enzyme-linked immunoassay (ELISA) with the phenolic glycolipid I to cross reference information and observe if they were or were not positive to this test. A positive ELISA indicates the bacillus is still present in the patient.</span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Results:</span> As an important fact, we found that of 78 patients cured, when bearing in mind the monitoring period, 9 were positive for the ELISA. When this period was discarded, 81 sick individuals were classified as cured according to WHO criteria but the same 9 continued positive for ELISA. </span><br /> <span style="font-family: Arial;"><span style="font-weight: bold;">Conclusion:</span> It may be concluded that in spite of meeting WHO criteria, these patients still show presence of the bacillus and the monitoring period is not required as a criterion to discharge a patient. We recommend adding to WHO criteria a negative ELISA, to obtain additional information that helps to certify that a patient is or is not cured.</span></small> ER -