Porphyromonas gingivalis Fim-A genotype distribution among Colombians
Abstract
Introducción:
Porphyromonas gingivalis es una bacteria asociadacon la periodontitis. Expresa una amplia gama de factores devirulencia, incluyendo las fimbrias, las cuales están codificadas porel gen FimA que representa seis genotipos conocidos.
Objetivo:
Identificar los genotipos de FimA de P. gingivalis enpacientes de Cali - Colombia , incluyendo la co -infección conAggregatibacter actinomycetemcomitans, Treponema denticola yTannerella forsythia.
Métodos:
Se obtuvieron muestras subgingivales de 151 individuoscon diferentes diagnósticos periodontales. La ocurrencia de P.gingivalis, los genotipos de FimA y otras bacterias se determinó porPCR.
Resultados:
Porphyromonas gingivalis fue positiva en 85 pacientes.El genotipo FimA II fue más prevalente, pero no hubo diferenciassignificativas entre los grupos de estudio (54.3%) , FimA IV fue elmás frecuente en la gingivitis (13.0%). Una alta correlación (p=0.000) se encontró entre P. gingivalis , T. denticola y T. forsythia.El genotipo FimA II estuvo correlacionado con la detección de T.denticola y T. forsythia.
Conclusiones:
Porphyromonas gingivalis tuvo una alta frecuenciaincluso en el grupo de individuos sanos. Se encontró una tendenciahacia una mayor frecuencia de FimA II en pacientes con periodontitismoderada y severa. El genotipo FimA II también se asoció con unamayor profundidad de la bolsa, una mayor pérdida de nivel deinserción, y con los pacientes en los que se detectó co-infección conT. denticola y T. forsythia
Authors
Downloads
Keywords
- Porphyromonas gingivalis
- Bacterial fimbriae
- Polymerase Chain Reaction
- periodontitis
- gingivitis.
References
Darveau R. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010; 8: 481-490.
Zijnge V, Van Leeuwen B, Degener J, et al. Oral biofilm architecture on natural teeth. Plos one. 2012, 5 (2): 9321
Dornelles F, Santos D, Van Dyke T, et al. In vivo up-regulation of kinin B1 receptors after treatment with Porphyromonas gingivalis lipopolysaccharide in rat paw. J Pharmacol Exp Ther. 2009; 33 (3): 756 -63.
Yilmaz O. The chronicles of Porphyromonas gingivalis: the microbium, the human oral epithelium and their interplay. Microbiol. 2008: 154: 2897– 903.
Holt SC, Kesavalu L, Walker S, Genco CA. Virulence factors of Porphyromonas gingivalis. Periodontol 2000. 1999; 20: 168 – 238.
Slots J, Ting M. Actinobacillus actinomycetemcomitans and Porphromonas gingivalis in human periodontal disease: occurrence and treatment. Periodontol 2000. 1999; 20: 82-121.
Brunner J, Scheres N, El Idrissi N, et al. The capsule of Porphyromonas gingivalis reduces the immune response of human gingival fibroblasts. BMC Microbiol. 2010; 10 (5) : 1471 – 82.
Stathopoulou P, Galicia J, Benakanakere M, et al. Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism. BMC Microbiol. 2009; 9:107- 119.
Wang M, Liang S, Hosur K, et al. Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis. Oral Microbiol Immunol. 2009; 24, (6): 478–84.
Inaba H, Nakano K, Kato T, et al. Heterogenic virulence and related factors among clinical isolated of Porphyromonas gingivalis with type II fimbriae. Oral Microbiol Immunol. 2008; 23: 29-35.
Teixeira S, Mattarazo F, Feres M, et al. Quantification of Porphyromonas gingivalis and FimA genotypes in smoker chronic periodontitis. J of Clin Per. 2009; 36:482-87.
Nakano K, Inaba H, Nomura R, et al.Distribution of Porphyromonas gingivalis FimA genotypes in cardiovascular specimens from Japanese patients. Oral Microbiol Immunol 2008; 23:170-2.
Pérez P, Lafaurie G, Gracieux P, et al. Distribution of Porphyromonas gingivalis FimA genotypes in isolates from subgingival plaque and blood sample during bacteremia. Biomédica. 2009; 29: 298-306.
Amano A, Nakagawa I, Kataoka K, Morisaki I, Amada S. Distribution of Porphyromonas gingivalis strains with FimA genotypes in periodontitis patients. J of Clin Microbiol. 1999; 37: 1426–30.
Amano A, Kuboniwa I, Nakagawa,et al. Prevalence of specific genotypes of Porphyromonas gingivalis FimA and periodontal health status. J Dent Res. 2000; 79: 1664-8.
Missailidis CG, Umeda JE, Ota-Tsuzuki C, Anzai D, Mayer MPA. Distribution of FimA genotypes of Porphyromonas gingivalis in subjects with various periodontal conditions. Oral Microbiol Immunol. 2004; 19: 22-9.
Davila-Pérez C, Amano A, Alpuche-Solis AG, et al. Distribution of genotypes of Porphyromonas gingivalis in type 2 diabetic patients with periodontitis in Mexico. J Clin Per. 2007; 34: 25–30.
Amano A, Kimura R, Nakamura T, et al. Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of FimA gene. J Clin Microbiol. 2000; 38:1909-14.
Guo YH, Wu YF, Liu TJ, et al. The distribution of FimA genotype of Porphyromonas gingivalis in chronic periodontitis patients. Hua Xi Kou Qiang Yi Xue Za Zhi. 2005; 33:99-102. ( in Chinesse).
Beikler T, Peters U, Prajaneh S, et al. Prevalence of Porphyromonas gingivalis FimA genotypes in caucasians. J Oral Sci. 2003; 111:390-4.
Van der Ploeg J, Giertsen E, Ludin B, et al. Quantitative detection of Porphyromonas gingivalis FimA genotypes in dental plaque. FEMS Microbiol Lett. 2004; 232:31-7.
Wu YF, Guo YH, Liu TJ, et al. Distribution of FimA genotype of Porphyromonas gingivalis in Chinese periodontitis patients and its relationship with chronic periodontitis. Sichuan Da Xue Xue Bao Yi Xue Ban, 2006; 37: 101-108. (in Chinesse).
Zhao L, Wu Y-F, Meng S, et al. Prevalence of FimA genotypes of Porphyromonas gingivalis and periodontal health status in chinese adults. J Per Res. 2007; 42: 511-17.
Botero JE, Contreras A, Lafaurie G, et al. Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. J of Per. 2007; 78, (4):696-704.
Lafaurie G, Contreras A, Barón A, et al. Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J of Per, 2007; 78: 629 -39.
Mayorga-Fayad I, Lafaurie GI, Contreras A, et al. Subgingival microbiota in chronic and aggressive periodontitis in Bogotá, Colombia: an epidemiological approach. Biomedica. 2007; 27, (1):21-33.
Moon JH, Shin S, Chung J, et al. Development and evaluation of new primers for PCR-based identification of type II fimA of Porphyromonas gingivalis. FEMS Immunol Med Microbiol 64 (2012) 425–428
Moon JH, Herr Y, Lee HW, et al. Genotype analysis of Porphyromonas gingivalis fimA in Korean adults using new primers. Journal of Medical Microbiology. 2013; 62: 1290–94
Enersen M, Olsen I, Kvalheim O, et al. FimA genotypes and multilocus sequence types of Porphyromonas gingivalis from patients with periodontitis. J Clin Microbiol. 2008; 46:31-42.
Nakagawa I, Amano A, Kuboniwa M, et al. Functional differences among FimA variants of Porphyromonas gingivalis and their effects on adhesion to and Invasion of human epithelial cells. Infect Immun. 2002; 70: 277-85.
Socransky S.S, Hafajee A. D, Dental biofilms: difficult therapeutic targets. Periodontol 2000. 2002; 28: 12–55.
Jaramillo A, Arce RM, Herrera D, et al. Clinical and microbiological characterization of periodontal abscesses. J Clin Per. 2005; 32, (12): 1213-8.
Copyright (c) 2015 Universidad del Valle
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The copy rights of the articles published in Colombia Médica belong to the Universidad del Valle. The contents of the articles that appear in the Journal are exclusively the responsibility of the authors and do not necessarily reflect the opinions of the Editorial Committee of the Journal. It is allowed to reproduce the material published in Colombia Médica without prior authorization for non-commercial use
