Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans
Abstract
- Introduction: Several studies have reported that the single nucleotide polymorphism rs693 of Apo lipoprotein B gene is associated with high levels of plasma lipids and high body mass index, which are risk factors for cardiovascular diseases. The distribution of this single nucleotide polymorphism and its association with the phenotype depend on the genetic background of each population.
- Objective: To evaluate the distribution of single nucleotide polymorphism rs693 and its association with lipid profile and body mass index in a sample of Colombian Caribbeans.
- Methods: 108 non-related adult subjects of both gender were included in this study. Body mass index and lipid profile that included total cholesterol, triglycerides, Low Density Lipoprotein and High Density Lipoprotein were determined. The single nucleotide polymorphism rs693 was determined by Polymerase Chain Reaction/Restriction Fragment Length Polymorphism from genomic DNA followed by digestion with the restriction enzyme XbaI. The chi-square test was used to analyze the genotype distribution of rs693 and the genotype-phenotype association was evaluated through different inheritance model.
- Results: The genotype frequencies for single nucleotide polymorphism rs693 were CC (45.0%), TT (16.5%) and CT (38.5%). The allele frequencies were C (64.0%) and T (36.0%). The single nucleotide polymorphism was in Hardy-Weinberg equilibrium in the studied sample. No association of the single nucleotide polymorphism rs693 with lipid profile nor the body mass index was found (p >0.05).
- Conclusion: There is no significant association between single nucleotide polymorphism rs693 and body mass index nor lipid profile, in a sample of Colombian Caribbeans.
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Keywords
- apolipoprotein B
- lipid profile
- body mass index
- polymorphism
- genotyping
Author Biographies
, Professor and Researcher. Faculty of Health Sciences. Universidad Libre, Barranquilla-Colombia.
Universidad Libre, Grupo de Investigación Avanzada en Biomedicina, Barranquilla, Colombia
https://orcid.org/0000-0002-4586-3753, Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
https://orcid.org/0000-0001-7431-4967, 1.Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.2. Universidad del Norte, Barranquilla, Colombia.
1-Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
2 Universidad del Norte, Barranquilla, Colombia.
https://orcid.org/0000-0002-7240-7462
, 1-Universidad Metropolitana, Grupo de Investigación en Medicina Traslacional (GIMET), Barranquilla, Colombia. 2- Corporación Universitaria Rafael Núñez, Programa de Enfermería, Barranquilla, Colombia.
1-Universidad Metropolitana, Grupo de Investigación en Medicina Traslacional (GIMET), Barranquilla, Colombia.
2- Corporación Universitaria Rafael Núñez, Programa de Enfermería, Barranquilla, Colombia.
https://orcid.org/0000-0003-3589-1547, Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
https://orcid.org/0000-0001-5270-2838, Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia.
https://orcid.org/0000-0001-6000-3113, Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia
Universidad Libre, Grupo de Investigación en Bioquímica Patológica (GRUBIOPAT). Barranquilla, Colombia
https://orcid.org/0000-0003-4018-146XReferences
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