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  1. Home /
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  3. Vol 43 No 3 (2012) /
  4. Original Articles

Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction

  • Abstract
  • Keywords
  • References

Abstract

 

Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and

pregnant women with normal pregnancies.

Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18

women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma

by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through

technical Real Time-Chain Reaction Polymerase.

Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically

significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA

expression of hPL gene (p = 0.5785) between cases and controls.

Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.

 

Authors

  • Paola Andrea Ayala Ramírez Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
  • Reggie García Robles Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
  • Juan Diego Rojas Barrera Unidad de Medicina Materno Fetal. Departamento de Ginecobstetricia, Hospital San Ignacio, Bogotá, D.C., Colombia
  • Martha Cecilia Bermúdez Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
  • Jaime Eduardo Bernal Villegas Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia

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Keywords

  • IUGR
  • messenger RNA
  • plasma
  • complications of pregnancy
  • human placental lactogen
  • Reverse Transcriptase Polymerase Chain Reaction

References

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Published
2012-09-28
Submitted
2011-09-12
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How to Cite
Ayala Ramírez, P., García Robles, R., Rojas Barrera, J., Bermúdez, M., & Bernal Villegas, J. (2012). Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction. Colombia Médica, 43(3), 184-188. https://doi.org/10.25100/cm.v43i3.863
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Vol 43 No 3 (2012)
Section
Original Articles

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