Effects of treatment with leptin-like peptides on factors related to body fat control in Wistar rats fed a high-fat diet*
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Objective: To demonstrate in Wistar rats fed with high-fat diet if the administration of synthetic peptides corresponding to the 116-130 peptide of mouse leptin (SR 116), its human homologue peptide (SH 95: sequence 95-109 from the 1AX8 protein) and five modified peptides (P80 to P84) similar to these two peptides, produces effects related to regulation of body fat.
Materials and methods: Nine-week old Wistar rats were fed a high-fat diet for fifteen weeks. On the fifteenth week, and for five consecutive days, they were treated with the peptides to be evaluated. During the days of treatment, body weight and food intake were evaluated. After the last peptide administration, lipid profile, glycerol in the cellular medium, and DNA fragmentation in adipocytes were analyzed.
Results: The results revealed that: the SR116 peptide affects the regulation of adiposity in rats fed a high-fat diet. The SH 95 is the human peptide with biological activity similar to SR 116 to lower weight, lessen food intake, and increase free glycerol in the cellular medium. The P80 and P81 peptides had a similar effect on SR 116 and SH 95 regarding body weight and food intake. The SR 116, SH 95 and three of the modified peptides (P80, P81, and P82) caused DNA fragmentation.
Conclusion: The results suggest that peptides analogous to leptin are potentially viable to achieve effects of adiposity reduction in Wistar rats with obesity associated to high-fat diet; more research is rendered to explain the differences among peptides and the biological action mechanisms.
- Grasas en la dieta
- Adiposidad
- Análogos de leptina
- Terapéutica
- Ratas Wistar
Ceddia RB. Direct metabolic regulation in skeletal muscle and
Colombia Médica Vol. 42 Nº 1, 2011 (Enero-Marzo)fat tissue by leptin: implications for glucose and fatty acidshomeostasis. Int J Obes (Lond). 2005; 29: 1175-83.2.Wang M, Lee Y, Unger R. Novel form of lipolysis induced byleptin. J Biol Chem. 1999; 25: 17541-4.3.Zhang H, Kumar S, Bernett A, Eggo M. Intrinsic site-specificdifferences in the expression of leptin in human adipocytesand its autocrine effects on glucose uptake. J Clin Endocrinol.1999; 7: 2550-5.4.Rodríguez VM, Macarulla, MT Echevarría, E, Portillo MP.Lipolysis induced by leptin in rat adipose tissue from differentanatomical locations. Eur J Nutr. 2003; 42: 149-53.5.Grasso P, Leinung MC, Ingher SP, Lee DW. In vivo effects ofleptin related synthetic peptides on body weight and foodintake in female ob/ob mice. Localization of leptin activity todomains between amino acid residues 106-140. Endocrinology.1997; 4: 1413-18.6.Rozhavskaya A, Lee D, Leinung M, Grasso P. Design of asynthetic leptin agonist. Effects on energy balance, glucosehomeostasis, and thermoregulation. Endocrinology. 2000; 7:2501-7.7.Grasso P, Rozhavskaya-Arena M, Leinung MC, Lee DW. D-Leu-4- OB3, a synthetic leptin agonist, improves hyperglycemiccontrol in C57BL/6jJ ob/ob mice. Regul Pept. 2001; 101: 123-9.8.Scarpace PJ, Zhang Y. Leptin resistance: a pre-disposingfactor for diet-induced obesity. Am J Physiol Regul IntegrComp Physiol. 2009; 3: 493-500.9.Enriori PJ, Evans AE, Sinnayah P, Cowley MA. Leptinresistance and obesity. Obesity. 2006; 14 (Suppl 5): 254-8.10. Plantenga M, Saris W, Hukshorn C, Campfield ?. Effects ofweekly administration of pegylated recombinant human OBprotein on appetite profile and energy metabolism. Am J ClinNutrition. 2001; 74: 426-34.11. Kamohara S, Burcelin R, Halaas J, Friedman J, Charron M.Acute stimulation of glucose metabolism in mice by leptintreatment. Nature. 1997; 6649: 374-7.12. Burcelin R, Kamohara S, Li J, Tannenbaum G, Charron M,Friedman J. Acute intravenous leptin infusion increases glucoseturnover but not skeletal muscle glucose uptake in ob/ob mice.Diabetes.1999; 48: 1264-9.13. Gullicksen PS, Asuman DB, Dean RG, Hartzell DL, Baile CA.Adipose tissue cellularity and apoptosis after intracerebro-ventricular injections of leptin and 21 days of recovery in rats.Int J Obesity. 2003; 27: 302-12.14. Kalaivanisailaja J, Manju V, Nalini N. Lipid profile in micefed a high-fat diet after exogenous leptin administration. PolJ Pharmacol. 2003; 5: 763-9.15. Poveda E, Lareo L. Diseño teórico de péptidos miméticos deleptina como farmacóforo para tratamiento de obesidad. LectNutr. 2001; 8: 40-52.16. Poveda E, Trujillo P, Ruiz F, López E. Glicemia y concentra-ciones de insulina en sangre de ratas Wistar sometidas a dietaalta en grasa y a tratamiento con péptidos miméticos deleptina. Biomedica. 2008; 28: 50-63.17. Qian H, Azain MJ, Hartzell DL, Baile CA. Increased leptinresistance as rats grow to maturity. Proc Soc Exp Biol Med.1998; 2: 160-5.18. Wang T, Hartzell DL, Rose BS, Flatt WP, Hulsey MG, MenonNK, et al. Metabolic responses to intracerebroventricularleptin and restricted feeding. Physiol Behav. 1999; 4-5: 839-48.19. Rodbell M. Metabolism of isolated fat cells. Effects ofhormones on glucose metabolism and lipolysis. J Biol Chem.1964; 2: 375-80.20. Della-Fera MA, Choi YH, Hartzell DL, Duan J, Hamrick M,Baile CA. Sensitivity of ob/ob mice to leptin-induced adiposetissue apoptosis. Obes Res. 2005; 9: 1540-7.21. Maedler K, Schulthess FT, Bielman C, Berney T, Bonny C,Prentki M, e t a l. Glucose and leptin induce apoptosis in humanbeta-cells and impair glucose-stimulated insulin secretionthrough activation of c-Jun N-terminal kinases. FASEB J.2008; 22: 1905-13.22. Ramsay TG. Porcine leptin inhibits lipogenesis in porcineadipocytes. J Anim Sci. 2003; 81: 3008-17.23. Sahu A. Leptin signalling in the hypothalamus: emphasis onenergy homeostasis and leptin resistance. Front Neuro-endocrinol. 2003; 24: 225-53.24. Shen J, Tanida M, Mtijima A, Nagaik. In vivo effects of leptinon autonomic nerve activity and lipolysis in rats. NeurosciLett. 2007; 416: 193-7.25. Denke MA. Dietary fats, fatty acids, and their effects onlipoproteins. Curr Atheroscler Rep. 2006; 6: 466-71
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