Porphyromonas gingivalis Fim-A genotype distribution among Colombians
Article Sidebar
Main Article Content
Introduction: Porphyromonas gingivalis is associated with
periodontitis and exhibit a wide array of virulence factors, including fimbriae which is encoded by the FimA gene representing six known genotypes.
Objetive: To identify FimA genotypes of P. gingivalis in subjects from Cali-Colombia, including the co-infection with Aggregatibacter actinomycetemcomitans, Treponema denticola, and Tannerella forsythia.
Methods: Subgingival samples were collected from 151 people exhibiting diverse periodontal condition. The occurrence of P. gingivalis, FimA genotypes and other bacteria was determined by PCR.
Results: Porphyromonas gingivalis was positive in 85 patients. Genotype FimA II was more prevalent without reach significant differences among study groups (54.3%), FimA IV was also prevalent in gingivitis (13.0%). A high correlation (p= 0.000) was found among P. gingivalis, T. denticola, and T. forsythia co-infection. The FimA II
genotype correlated with concomitant detection of T. denticola and T. forsythia.
Conclusions: Porphyromonas gingivalis was high even in the healthy group at the study population. A trend toward a greater frequency of FimA II genotype in patients with moderate and severe periodontitis was determined. The FimA II genotype was also associated with increased pocket depth, greater loss of attachment level, and patients co-infected with T. denticola and T. forsythia.
- Porphyromonas gingivalis
- Bacterial fimbriae
- Polymerase Chain Reaction
- periodontitis
- gingivitis.
Darveau R. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010; 8: 481-490. DOI: https://doi.org/10.1038/nrmicro2337
Zijnge V, Van Leeuwen B, Degener J, et al. Oral biofilm architecture on natural teeth. Plos one. 2012, 5 (2): 9321 DOI: https://doi.org/10.1371/journal.pone.0009321
Dornelles F, Santos D, Van Dyke T, et al. In vivo up-regulation of kinin B1 receptors after treatment with Porphyromonas gingivalis lipopolysaccharide in rat paw. J Pharmacol Exp Ther. 2009; 33 (3): 756 -63. DOI: https://doi.org/10.1124/jpet.109.155762
Yilmaz O. The chronicles of Porphyromonas gingivalis: the microbium, the human oral epithelium and their interplay. Microbiol. 2008: 154: 2897– 903. DOI: https://doi.org/10.1099/mic.0.2008/021220-0
Holt SC, Kesavalu L, Walker S, Genco CA. Virulence factors of Porphyromonas gingivalis. Periodontol 2000. 1999; 20: 168 – 238. DOI: https://doi.org/10.1111/j.1600-0757.1999.tb00162.x
Slots J, Ting M. Actinobacillus actinomycetemcomitans and Porphromonas gingivalis in human periodontal disease: occurrence and treatment. Periodontol 2000. 1999; 20: 82-121. DOI: https://doi.org/10.1111/j.1600-0757.1999.tb00159.x
Brunner J, Scheres N, El Idrissi N, et al. The capsule of Porphyromonas gingivalis reduces the immune response of human gingival fibroblasts. BMC Microbiol. 2010; 10 (5) : 1471 – 82. DOI: https://doi.org/10.1186/1471-2180-10-5
Stathopoulou P, Galicia J, Benakanakere M, et al. Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism. BMC Microbiol. 2009; 9:107- 119. DOI: https://doi.org/10.1186/1471-2180-9-107
Wang M, Liang S, Hosur K, et al. Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis. Oral Microbiol Immunol. 2009; 24, (6): 478–84. DOI: https://doi.org/10.1111/j.1399-302X.2009.00545.x
Inaba H, Nakano K, Kato T, et al. Heterogenic virulence and related factors among clinical isolated of Porphyromonas gingivalis with type II fimbriae. Oral Microbiol Immunol. 2008; 23: 29-35. DOI: https://doi.org/10.1111/j.1399-302X.2007.00386.x
Teixeira S, Mattarazo F, Feres M, et al. Quantification of Porphyromonas gingivalis and FimA genotypes in smoker chronic periodontitis. J of Clin Per. 2009; 36:482-87. DOI: https://doi.org/10.1111/j.1600-051X.2009.01411.x
Nakano K, Inaba H, Nomura R, et al.Distribution of Porphyromonas gingivalis FimA genotypes in cardiovascular specimens from Japanese patients. Oral Microbiol Immunol 2008; 23:170-2. DOI: https://doi.org/10.1111/j.1399-302X.2007.00406.x
Pérez P, Lafaurie G, Gracieux P, et al. Distribution of Porphyromonas gingivalis FimA genotypes in isolates from subgingival plaque and blood sample during bacteremia. Biomédica. 2009; 29: 298-306. DOI: https://doi.org/10.7705/biomedica.v29i2.31
Amano A, Nakagawa I, Kataoka K, Morisaki I, Amada S. Distribution of Porphyromonas gingivalis strains with FimA genotypes in periodontitis patients. J of Clin Microbiol. 1999; 37: 1426–30. DOI: https://doi.org/10.1128/JCM.37.5.1426-1430.1999
Amano A, Kuboniwa I, Nakagawa,et al. Prevalence of specific genotypes of Porphyromonas gingivalis FimA and periodontal health status. J Dent Res. 2000; 79: 1664-8. DOI: https://doi.org/10.1177/00220345000790090501
Missailidis CG, Umeda JE, Ota-Tsuzuki C, Anzai D, Mayer MPA. Distribution of FimA genotypes of Porphyromonas gingivalis in subjects with various periodontal conditions. Oral Microbiol Immunol. 2004; 19: 22-9. DOI: https://doi.org/10.1111/j.1399-302X.2004.00140.x
Davila-Pérez C, Amano A, Alpuche-Solis AG, et al. Distribution of genotypes of Porphyromonas gingivalis in type 2 diabetic patients with periodontitis in Mexico. J Clin Per. 2007; 34: 25–30. DOI: https://doi.org/10.1111/j.1600-051X.2006.01011.x
Amano A, Kimura R, Nakamura T, et al. Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of FimA gene. J Clin Microbiol. 2000; 38:1909-14. DOI: https://doi.org/10.1128/JCM.38.5.1909-1914.2000
Guo YH, Wu YF, Liu TJ, et al. The distribution of FimA genotype of Porphyromonas gingivalis in chronic periodontitis patients. Hua Xi Kou Qiang Yi Xue Za Zhi. 2005; 33:99-102. ( in Chinesse).
Beikler T, Peters U, Prajaneh S, et al. Prevalence of Porphyromonas gingivalis FimA genotypes in caucasians. J Oral Sci. 2003; 111:390-4. DOI: https://doi.org/10.1034/j.1600-0722.2003.00065.x
Van der Ploeg J, Giertsen E, Ludin B, et al. Quantitative detection of Porphyromonas gingivalis FimA genotypes in dental plaque. FEMS Microbiol Lett. 2004; 232:31-7. DOI: https://doi.org/10.1016/S0378-1097(04)00064-3
Wu YF, Guo YH, Liu TJ, et al. Distribution of FimA genotype of Porphyromonas gingivalis in Chinese periodontitis patients and its relationship with chronic periodontitis. Sichuan Da Xue Xue Bao Yi Xue Ban, 2006; 37: 101-108. (in Chinesse).
Zhao L, Wu Y-F, Meng S, et al. Prevalence of FimA genotypes of Porphyromonas gingivalis and periodontal health status in chinese adults. J Per Res. 2007; 42: 511-17. DOI: https://doi.org/10.1111/j.1600-0765.2007.00975.x
Botero JE, Contreras A, Lafaurie G, et al. Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. J of Per. 2007; 78, (4):696-704. DOI: https://doi.org/10.1902/jop.2007.060129
Lafaurie G, Contreras A, Barón A, et al. Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J of Per, 2007; 78: 629 -39. DOI: https://doi.org/10.1902/jop.2007.060187
Mayorga-Fayad I, Lafaurie GI, Contreras A, et al. Subgingival microbiota in chronic and aggressive periodontitis in Bogotá, Colombia: an epidemiological approach. Biomedica. 2007; 27, (1):21-33. DOI: https://doi.org/10.7705/biomedica.v27i1.230
Moon JH, Shin S, Chung J, et al. Development and evaluation of new primers for PCR-based identification of type II fimA of Porphyromonas gingivalis. FEMS Immunol Med Microbiol 64 (2012) 425–428 DOI: https://doi.org/10.1111/j.1574-695X.2011.00889.x
Moon JH, Herr Y, Lee HW, et al. Genotype analysis of Porphyromonas gingivalis fimA in Korean adults using new primers. Journal of Medical Microbiology. 2013; 62: 1290–94 DOI: https://doi.org/10.1099/jmm.0.054247-0
Enersen M, Olsen I, Kvalheim O, et al. FimA genotypes and multilocus sequence types of Porphyromonas gingivalis from patients with periodontitis. J Clin Microbiol. 2008; 46:31-42. DOI: https://doi.org/10.1128/JCM.00986-07
Nakagawa I, Amano A, Kuboniwa M, et al. Functional differences among FimA variants of Porphyromonas gingivalis and their effects on adhesion to and Invasion of human epithelial cells. Infect Immun. 2002; 70: 277-85. DOI: https://doi.org/10.1128/IAI.70.1.277-285.2002
Socransky S.S, Hafajee A. D, Dental biofilms: difficult therapeutic targets. Periodontol 2000. 2002; 28: 12–55. DOI: https://doi.org/10.1034/j.1600-0757.2002.280102.x
Jaramillo A, Arce RM, Herrera D, et al. Clinical and microbiological characterization of periodontal abscesses. J Clin Per. 2005; 32, (12): 1213-8. DOI: https://doi.org/10.1111/j.1600-051X.2005.00839.x
Downloads
- Marisol Betancourth, Javier Enrique Botero, Sandra Patricia Rivera, Biopelículas: una comunidad microscópica en desarrollo. , Colombia Medica: Vol. 35 No. 3.Supl.1 (2004)
- Andres F Zea-Vera, Mario Alejandro Chacón, MD, Beatriz Parra, Antibody deficiencies with normal IgG in adults with Non-cystic fibrosis bronchiectasis or recurrent pneumonia: Cross-sectional study , Colombia Medica: Vol. 53 No. 2 (2022)
- Juan Fernando Aristizabal, Hector Rios, Diego Rey, Maria Antonia Alvarez, Beatriz Parra, Mario Ortiz, Relationship between the polymorphism in the interleukin 1-β and the treatment time of patients subjected to a modified piezocision technique , Colombia Medica: Vol. 51 No. 1 (2020)
- Javier Enrique Botero, Adriana Posada-López, Jimi Mejía-Vallejo, Ricardo Antonio Pineda-Tamayo, Emilio Bedoya-Giraldo, Effects of nonsurgical periodontal therapy in patients with rheumatoid arthritis: a prospective before and after study , Colombia Medica: Vol. 52 No. 3 (2021)
- Marisol Betancourth, Roger Mauricio Arce, Javier Botero, Adriana Jaramillo, Carlos Cruz, Adolfo Contreras, Microorganismos inusuales en surcos y bolsas periodontales. , Colombia Medica: Vol. 37 No. 1 (2006)
- Julián A. Herrera, Santiago Vélez Medina, Rodolfo Molano, Virna Medina, Javier E. Botero, Beatriz Parra, Adolfo Contreras, Periodontal intervention effects on pregnancy outcomes in women with preeclampsia. , Colombia Medica: Vol. 40 No. 2 (2009)
- Adriana Jaramillo, Gloria Inés Lafaurie, Lina Viviana Millán, Carlos Martin Ardila, Andrés Duque, Camilo Novoa, Diego López, Adolfo Contreras, Association between periodontal disease and plasma levels of cholesterol and triglycerides , Colombia Medica: Vol. 44 No. 2 (2013)
- Freddy Moreno, Sandra Milena Moreno, Carlos Andrés Díaz, Edwin Alexander Bustos, José Vicente Rodríguez, Prevalencia y variabilidad de ocho rasgos morfológicos dentales en jóvenes de tres colegios de Cali, 2002. , Colombia Medica: Vol. 35 No. 3.Supl.1 (2004)
- Beatriz Parra, Isabella Borrero, Felipe García, Caracterización de las glicoproteínas de una cepa de virus respiratorio sincicial aislada en Cali. , Colombia Medica: Vol. 28 No. 2 (1997)
- Sandra M Moreno, Adolfo Contreras, Functional differences of Porphyromonas gingivalis fimbriae's determining periodontal disease pathogenesis: A Literature Review , Colombia Medica: Vol. 44 No. 1 (2013)
Similar Articles
- Sandra M Moreno, Adolfo Contreras, Functional differences of Porphyromonas gingivalis fimbriae's determining periodontal disease pathogenesis: A Literature Review , Colombia Medica: Vol. 44 No. 1 (2013)
You may also start an advanced similarity search for this article.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The copy rights of the articles published in Colombia Médica belong to the Universidad del Valle. The contents of the articles that appear in the Journal are exclusively the responsibility of the authors and do not necessarily reflect the opinions of the Editorial Committee of the Journal. It is allowed to reproduce the material published in Colombia Médica without prior authorization for non-commercial use