NAT2 gene polymorphisms in three indigenous groups in the Colombian Caribbean Coast region

https://doi.org/10.25100/cm.v45i4.1563

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Published: Dec 28, 2014
Updated: 2014-12-28
Issue: Vol. 45 No. 4 (2014)
Section Original Articles

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Authors

Isis Arias Universidad del Norte, Barranquilla
Nelly Lecompte Universidad del Norte, Barranquilla
Lila Visbal Universidad del Norte, Barranquilla
Iliana Curiel Universidad del Norte, Barranquilla
Enio Hernández Universidad Cooperativa de Colombia sede Santa Marta
Pilar Garavito Universidad del Norte, Barranquilla
Carlos Silvera-Redondo Universidad del Norte, Barranquilla
Abstract

Objective: To study the NAT2 gene polymorphisms 481T, 590A, and 857A in the Chimila, Wiwa, and Wayuu Indigenous groups of the Colombian Caribbean to determine the frequencies of the allelesNAT2*4, NAT2*5, NAT2*6, and NAT2*7 and to determine the types of acetylation present in these populations.



Methods: A total of 202 subjects were studied: 47 Chimila, 55 Wiwa, and 100 Wayuu. The polymorphisms were identified using a real-time PCR method for allelic discrimination designed using Taqman of Applied Biosystems.

Results: The following alleles were found at the highest frequency in the following groups: the NAT2*4 allele (wild type) in the Wayuu group (55.3%), the NAT2*5 allele in the Wiwa group (34.5%), and the NAT2*7 allele in the Chimila group (24.2%). A higher frequency of the rapid acetylation status was found in the Wayuu group (31.3%) and Chimila group (29.5%) compared with the Wiwa group (12.7%). The intermediate acetylation status distribution was very similar in all three groups, and the frequency of the slow acetylation status was higher in the Wiwa group (32.7%) compared with the Chimila and Wayuu groups (20.5% and 21.2%, respectively).

Conclusion: The results demonstrated the allelic distribution and pharmacogenetic differences of the three groups studied and revealed the most frequent acetylation status and phenotype. Because of the high prevalence of slow acetylations, a greater incidence of tuberculosis (TB) drug-induced hepatotoxicity is predicted in these populations, with a higher frequency in the Wiwa group.

 

Keywords
  • NAT2
  • single nucleotide polymorphism
  • genotyping
  • acetylation
  • Isoniazid
  • Chimila
  • Wiwa
  • Wayuu
  • Indigenous groups
Author Biographies

Isis Arias, Universidad del Norte, Barranquilla

Grupo de Investigación en Genética y Medicina Molecular, Departamento de Medicina, Division Ciencia de la Salud

Nelly Lecompte, Universidad del Norte, Barranquilla

CINPE-Centro de Investigación en Neonatología y Pediatría, Departamento de Medicina, Division cienciad de la Salud

Lila Visbal, Universidad del Norte, Barranquilla

CINPE-Centro de Investigación en Neonatología y Pediatría, Departamento de Medicina, Division cienciad de la Salud

Iliana Curiel, Universidad del Norte, Barranquilla

CINPE-Centro de Investigación en Neonatología y Pediatría, Departamento de Medicina, Division cienciad de la Salud

Enio Hernández, Universidad Cooperativa de Colombia sede Santa Marta

Grupo de Investigación en Biomedicina Molecular, Facultad de Medicina

Pilar Garavito, Universidad del Norte, Barranquilla

Grupo de Investigación en Genética y Medicina Molecular, Departamento de Medicina, Division Ciencia de la Salud

Carlos Silvera-Redondo, Universidad del Norte, Barranquilla

Grupo de Investigación en Genética y Medicina Molecular, Departamento de Medicina, Division Ciencia de la Salud
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How to Cite
Arias, I., Lecompte, N., Visbal, L., Curiel, I., Hernández, E., Garavito, P., & Silvera-Redondo, C. (2014). NAT2 gene polymorphisms in three indigenous groups in the Colombian Caribbean Coast region. Colombia Medica, 45(4), 148–153. https://doi.org/10.25100/cm.v45i4.1563
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