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Authors

Objective:

To evaluate transcallosal changes after a local ischemic injury in rats by using the monoclonal marker anti-NeuN (Mouse anti-neuronal nuclei).

Methods:

Twenty eight adult, male, Wistar rats were subjected to focal injury in the right hemisphere. The technique used was the experimental model of focal ischemic injury through intraluminal suture of the middle cerebral artery. Analyses were made for the five groups: and after the lesion (control), at 24 hours, 96 hours, 10 days and 20 days. Exofocal neuronal damage was inferred from neuronal immunoreactivity changes to NeuN.

Results:

In the cortex contralateral to the lesion, immunoreactivity was diminished. This was most notable in the supragranular layers 24 hours post ischemia. After 96 hours, there was a generalized diminishment of the inmmunoreactivity in supra and infragranular layers. At 10 and 20 days, the tissue recovered some NeuN immunoreactivity, but there were set changes in the VI layer.

Conclusion:

The immunoreactive changes to NeuN support the process of interhemispheric diaschisis. Changes in immunoreactivity could indicate metabolic stress secondary to the disruption in connectivity to the site of lesion.

 

Laura Potes, Universidad Icesi, Cali, Colombia. Fundación Valle del Lili, Cali, Colombia.

Grupo de Investigación Biomédica Universidad ICESI, Cali, Colombia.

John Umbarila, Fundación Innovación y Ciencia por Colombia –FICC-, Bogotá, Colombia.

Centro de Investigación, Innovación y Desarrollo –CIID- Fundación Innovación y Ciencia por Colombia –FICC-, Bogotá, Colombia.

Cesar Augusto Arango-Dávila, Fundación Valle del Lili

-Fundación Valle del Lili

-Grupo de Investigación Biomédica Universidad ICESI, Cali,

Colombia.

Beatriz E Munoz, Pontificia Universidad Javeriana Cali - Colombia

Grupo Medición y Evaluación Psicológica Pontificia Universidad Javeriana, Cali, Colombia

Daniel Manrique Castaño, Pontificia Universidad Javeriana Cali - Colombia

Grupo Medición y Evaluación Psicológica Pontificia Universidad Javeriana, Cali, Colombia.

Centro de Investigación, Innovación y Desarrollo –CIID- Fundación Innovación y Ciencia por Colombia –FICC-, Bogotá, Colombia.

Potes, L., Umbarila, J., Arango-Dávila, C. A., Munoz, B. E., & Manrique Castaño, D. (2024). Assessment transcallosal Diaschisis in a model of focal cerebral ischemia in rats. Colombia Medica, 47(2), 87–93. https://doi.org/10.25100/cm.v47i2.2146 (Original work published June 13, 2016)

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Received 2015-11-05
Accepted 2016-05-19
Published 2024-06-07