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Introduction: Periapical changes named as lesions, in teeth with full crown integrity and without history of trauma, do not show a clear aetiology.
Objective: To determine the presence of microorganisms in pulp dental tissue will clarify the cause of its death and therefore the damage to periodontal tissues.
Materials and methods: From people between 10 and 39 years old, 23 teeth were selected. The samples were taken with paper points and 0.8 sterile files, and were transported in VMGA III medium, to be processed in the following 24 hours after they were taken and sowed in Brucella-agar.
Results: The most affected teeth were upper central incisors, 43.8%. From the 23 studied teeth, microbiological grow was seen on 20 teeth. The following microorganisms species were identified: Fusobacterium spp., 25%, Eubacterium spp., 15%; Peptostreptococcus spp., 10%; Campylobacter spp., 10%; gram negative enteric bacteria, 10%; Porphyromonas gingivalis, 10%; Prevotella intermedia, 5%; Eikenellia corrodens, 5%; Dialister pneumosintes, 5%; and yeasts, 5%. There was no growing evidence of Actinomyces actinomycetemcomitans, Tanerella forsythensis and Streptococcus β  hemolytic.
Discussion and conclusions: Sound pulp dental tissue is sterile; an injury over it will cause its inflammation, degeneration, death and bacterial contamination. Results in the present study clearly show the presence of microorganisms in closed apical dental lesions of endodontic origin. In same manner, it was seen that a great part of microorganisms species found can be regarded as periodontal pathogens. This could suggest a management with an endodontic, a periodontic and a pharmacological combined treatment.

Patricia Rodríguez, Universidad del Valle

Profesora Auxiliar, Escuela de Odontología, Facultad de Salud, Universidad del Valle, Cali.

Jesús Alberto Calero, Universidad del Valle

Profesor Asistente, Escuela de Odontología, Facultad de Salud, Universidad del Valle, Cali.
Rodríguez, P., & Calero, J. A. (2008). Pulp microbiology of complete teeth with idiopathic apical lesions. Colombia Médica, 39(1 Supl 1), 5–10. Supl 1.560


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