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Introduction: Cerebral ischemia is an important cause of brain lesion in humans. The target in research has been the ischemic core or the penumbra zones; little attention has been given to areas outside the core or the penumbra but connected with the primary site of injury.
Objective: Evaluate the laminar response of a subpopulation of gabaergic cells, those that are parvalbumin (PV) positive and the astrocytes through the expression of the glial transporter GLT1 on the contralateral cortex to an ischemic core.
Methodology:  For this purpose we used the medial cerebral artery occlusion model in rats. The artery was occluded for 90 minutes and the animals were sacrificed at 24 and 72 hours post-ischemia. The brains were removed, cut in a vibratome at 50 microns and incubated with the primary antibodies against PV or GLT1. Sections were developed using the vectastain Kit. In control tissue the primary antibody was omitted.
Results: When compared with control animals, treated ones show a decrease in the expression of  GLT1, especially in layers III and IV of the contralateral cortex to the ischemic core. PV positive cells increases in layers II and V.
Conclusion: Increases in the expression of PV cells could correspond to an adaptation associated with glutamate increases in the synaptic compartment. These increases may be due to decreases in the expression of GLT1 transporter, that could not remove the glutamate present in the synaptic cleft, generating hyperactivity in the contralateral cortex. These changes could represent an example of neuronal and glial plasticity in remote areas to an ischemic core but connected to the primary site of injury.

Adriana M. Medina, Universidad del Valle

Centro de Estudios Cerebrales, Facultad de Salud, Universidad del Valle; Cali, Colombia.

Neuroscience Laboratory, Michigan University, Ann Arbor, USA.

Norberto García Cairasco, Universidade de São Paulo

Laboratorio de Neurofisiologia e Neuroetologia Experimental, Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brasil.

Martha Isabel Escobar, Universidad del Valle

Centro de Estudios Cerebrales, Facultad de Salud, Universidad del Valle; Cali, Colombia.
Medina, A. M., García Cairasco, N., & Escobar, M. I. (2008). Contralateral cortical response of a subpopulation of interneurons and the glial glutamate transporter GLT1 to an ischemic core. Colombia Medica, 39(3 Supl 3), 14–24.


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