Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction

https://doi.org/10.25100/cm.v43i3.863

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Published: Sep 28, 2012
Updated: 2012-09-28
Issue: Vol. 43 No. 3 (2012)
Section Original Articles

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Authors

Paola Andrea Ayala Ramírez Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
Reggie García Robles Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
Juan Diego Rojas Barrera Unidad de Medicina Materno Fetal. Departamento de Ginecobstetricia, Hospital San Ignacio, Bogotá, D.C., Colombia
Martha Cecilia Bermúdez Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
Jaime Eduardo Bernal Villegas Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
Abstract

Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies.


Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase.


Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA expression of hPL gene (p = 0.5785) between cases and controls.


Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.

Keywords
  • IUGR
  • messenger RNA
  • plasma
  • complications of pregnancy
  • human placental lactogen
  • Reverse Transcriptase Polymerase Chain Reaction
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How to Cite
Ayala Ramírez, P. A., García Robles, R., Rojas Barrera, J. D., Bermúdez, M. C., & Bernal Villegas, J. E. (2012). Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction. Colombia Medica, 43(3), 184–188. https://doi.org/10.25100/cm.v43i3.863
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