Skip to main content Skip to main navigation menu Skip to site footer
Page Header Logo
  • ABOUT THE JOURNAL
    • Focus and scope
    • Editorial Management Process
  • EDITORIAL POLICY
    • Ethical Publication Standards
    • Open Access Policy
    • Anti-plagiarism Policy
    • Copyright
    • Policy for Journal Archiving
    • Claim Policy
  • AUTHORS
    • Guidelines to Authors
    • Sending and Receiving Papers
    • Structure and Content of Articles
    • Article Evaluation Process
    • ORCID
    • coi_disclosure Colombia Médica ICMJE
    • Copyright Transfer Statement Colombia Médica
  • PEER REVIEW
    • Peer Review Process
    • Evaluation Forms
  • EDITORIAL TEAM
    • Editorial team
    • Contact
  • ARTICLES
    • Current
    • Archives
  • COLLECTIONS
Search
  • Register
  • Login
  1. Home /
  2. Archives /
  3. Vol 48 No 2 (2017) /
  4. Original Articles

Real-time PCR assay for the diagnosis of pleural tuberculosis

  • Abstract
  • Keywords
  • Author Biographies
  • References

Abstract

Introduction:  The diagnosis of pleural tuberculosis requires an invasive and time-consuming reference method. Polymerase chain reaction (PCR) is rapid, but validation in pleural tuberculosis is still weak.Objective:  To establish the operating characteristics of real-time polymerase chain reaction (RT-PCR) hybridization probes for the diagnosis of pleural tuberculosis.Method: The validity of the RT-PCR hybridization probes was evaluated compared to a composite reference method by a cross-sectional study at the Hospital Universitario de la Samaritana. 40 adults with lymphocytic pleural effusion were included. Pleural tuberculosis was confirmed (in 9 patients) if the patient had at least one of three tests using the positive reference method: Ziehl-Neelsen or Mycobacterium tuberculosis culture in fluid or pleural tissue, or pleural biopsy with granulomas. Pleural tuberculosis was ruled out (in 31 patients) if all three tests were negative. The operating characteristics of the RT-PCR, using the Mid-P Exact Test, were determined using the OpenEpi 2.3 Software (2009).Results:  The RT-PCR hybridization probes showed a sensitivity of 66.7% (95% CI: 33.2%-90.7%) and a specificity of 93.5% (95% CI: 80.3%-98.9%). The PPV was 75.0% (95% CI: 38.8%-95.6%) and a NPV of 90.6% (95% CI: 76.6%-97.6%). Two false positives were found for the test, one with pleural mesothelioma and the other with chronic pleuritis with mesothelial hyperplasia.Conclusions: The RT-PCR hybridization probes had good specificity and acceptable sensitivity, but a negative value cannot rule out pleural tuberculosis.

Authors

  • Martha Alejandra Casallas-Rivera 1 Universidad de la Sabana. Chía, Colombia 2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia
  • Ana María Cardenas Bernal 1 Universidad de la Sabana. Chía, Colombia 2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia
  • Luis Fernando Giraldo-Cadavid Universidad de la Sabana. Chía, Colombia
  • Enrique Prieto Diago Hospital Universitario de la Samaritana. Bogotá, Colombia
  • Paola Santander Hospital Universitario de la Samaritana. Bogotá, Colombia

Downloads

Download data is not yet available.

Keywords

  • Diagnostic Tests
  • pleural effusion
  • Mycobacterium tuberculosis
  • tuberculosis pleural
  • validation studies
  • Real-Time Polymerase Chain Reaction
  • Ziehl-Neelsen
  • likelihood ratios

Author Biographies


, 1 Universidad de la Sabana. Chía, Colombia 2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia

1 Universidad de la Sabana. Chía, Colombia

2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia


, 1 Universidad de la Sabana. Chía, Colombia 2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia

1 Universidad de la Sabana. Chía, Colombia

2 Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia


, Universidad de la Sabana. Chía, Colombia
Universidad de la Sabana. Chía, Colombia

, Hospital Universitario de la Samaritana. Bogotá, Colombia
Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia

, Hospital Universitario de la Samaritana. Bogotá, Colombia
Internal Medicine, Hospital Universitario de la Samaritana. Bogotá, Colombia

References

Arnadottir Th. Tuberculosis and Public Health. Policy and Principles in Tuberculosis Control. Paris, France: International Union Against Tuberculosis and Lung Disease; 2009.

Dam T, Bose M. Paucibacillary tuberculosis a retrospective study. J Indian Med Assoc. 2002; 100(4): 231–3.

World Health Organization. Global Tuberculosis Report 2014. Geneva, Switzerland: WHO; 2014.

World Health Organization. Global Tuberculosis Report 2016. Geneva, Switzerland: WHO; 2016.

Instituto Nacional de Salud Comportamiento de los eventos de vigilancia en salud pública. Tuberculosis. Boletín Epidemiológico Semanal. 2015; Semana epidemiologica numero 52: 51–53.

Trajman A, Pai M, Dheda K, van Zyl SR, Zwerling AA, Joshi R. Novel tests for diagnosing tuberculous pleural effusion what works and what does not? Eur Respir. 2008; 31(5): 1098–106.

Simner PJ, Buckwalter SP, Uhl JR, Wengenack NL. Identification of Mycobacterium species and Mycobacterium tuberculosis complex resistance determinants by use of PCR-electrospray ionization mass spectrometry. J Clin Microbiol. 2013; 51(11): 3492–8.

Villena V, Lopez-Encuentra A, Pozo F, Echave-Sustaeta J, Ortuno-de-Solo B, Estenoz-Alfaro J. Interferon gamma levels in pleural fluid for the diagnosis of tuberculosis. Am J Med. 2003; 115(5): 365–70.

Scharer L, McClement JH. Isolation of tubercle bacilli from needle biopsy specimens of parietal pleura. Am Rev Respir Dis. 1968; 97(3): 466–8.

Lamsal M, Gautam N, Bhatta N, Majhi S, Baral N, Bhattacharya SK. Diagnostic utility of adenosine deaminase (ADA) activity in pleural fluid and serum of tuberculous and non-tuberculous respiratory disease patients. Southeast Asian J Trop Med Public Health. 2007; 38(2): 363–9.

Costa J. Reacción en cadena de la polimerasa (PCR) a tiempo real. Enfermedades Infecciosas y Microbiología Clínica. 2004; 22(5): 299–305.

Pai M, Flores LL, Hubbard A, Riley LW, Colford JM Jr. Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis a systematic review and meta-analysis. BMC Infect Dis. 2004;4:6–6.

Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol. 2005; 43(9): 4357–62.

Liu KT, Su WJ, Perng RP. Clinical utility of polymerase chain reaction for diagnosis of smear-negative pleural tuberculosis. J Chinese Med Assoc. 2007; 70(4): 148–51.

Friedrich SO, von Groote-Bidlingmaier F, Diacon AH. Xpert MTB/RIF assay for diagnosis of pleural tuberculosis. J Clin Microbiol. 2011; 49(12): 4341–2.

Kalantri Y, Hemvani N, Chitnis DS. Evaluation of real-time polymerase chain reaction, interferon-gamma, adenosine deaminase, and immunoglobulin A for the efficient diagnosis of pleural tuberculosis. Int J Infect Dis. 2011; 15(4): e226–31.

Rosso F, Michelon CT, Sperhacke RD, Verza M, Olival L, Conde MB. Evaluation of real-time PCR of patient pleural effusion for diagnosis of tuberculosis. BMC Res Notes. 2011; 4: 279.

Light RW. Clinical practice Pleural effusion. N Engl J Med. 2002; 346(25): 1971–7.

Duffau T. G Tamaño muestral en estudios sobre pruebas diagnósticas. Rev Chilena Pediatría. 1998; 69(3): 122–5.

Machin D, Campbell MJ, Beng TS, Huey TS. Sample Size Tables for Clinical Studies. Tercera edición ed: Wiley-Blackwell; 2009.

Li J, Fine J. On sample size for sensitivity and specificity in prospective diagnostic accuracy studies. Stat Med. 2004; 23(16): 2537–50.

  • PDF
  • PDF (Español (España))
  • Full Text
  • HTML (Español (España))
Published
2017-06-28
Submitted
2016-08-07
| 543 |
How to Cite
Casallas-Rivera, M., Cardenas Bernal, A., Giraldo-Cadavid, L., Prieto Diago, E., & Santander, P. (2017). Real-time PCR assay for the diagnosis of pleural tuberculosis. Colombia Médica, 48(2), 47-52. https://doi.org/10.25100/cm.v48i2.2471
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
Download Citation
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX
Issue
Vol 48 No 2 (2017)
Section
Original Articles

Copyright (c) 2017 Universidad del Valle

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The copy rights of the articles published in Colombia Médica belong to the Universidad del Valle. The contents of the articles that appear in the Journal are exclusively the responsibility of the authors and do not necessarily reflect the opinions of the Editorial Committee of the Journal. It is allowed to reproduce the material published in Colombia Médica without prior authorization for non-commercial use

Online ISSN: 1657-9534
Make a Submission

Bibliographics database

Full-text database

Citation Index

Bibliographical information system

Memberships

Licencia Creative Commons
This work is under License Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) .

Indexed

.
0.82
2018CiteScore
 
 
68th percentile
Powered by  Scopus
.
Information
  • For Authors
Universidad del Valle
Universidad del Valle
  • Cali - Colombia
  • © 1994 - 2020
Dirección:
  • Ciudad Universitaria Meléndez
  • Calle 13 # 100-00
  •  
  • Sede San Fernando
  • Calle 4B N° 36-00
PBX:
  • +57 2 3212100
  • Línea gratuita: 018000 22 00 21
  • A.A.25360
Redes Sociales:

2020 Universidad del Valle - Vigilada MinEducación

//Go to www.addthis.com/dashboard to customize your tools