Antracol WP 70 genotoxicity in human lymphocyte cultures.

Abstract

Introduction: Antracol WP 70 is a pesticide of common use in farming activities to mitigate pathologies in plants associated to mushrooms. In spite of its great application spectrum and incorrect manipulation, there were no genotoxicity studies found, carried out on human beings regarding this compound.
Aim: To evaluate, by means of structural chromosomal aberration test (SCA), the genotoxicity effect of Antracol WP 70 in human lymphocyte cultures of peripheral blood.
Materials and methods: Human lymphocyte cultures were established in RPMI 1640 supplemented Medium and incubated at 37° C. The cultures were treated with sterile distilled water (control), high concentration of the pesticide (2.5 mg/ml), half (1.25 mg/ml) and low (0.02 mg/ml), during a 48 hours period. The metaphase cells were evaluated and the SCA observed were registered and analyzed to estimate the respective statistical values.
Results: The biggest number of SCA was found in cells tried with the half concentration and the lowest one was for the group control. However, there were no statistically significant differences found at a confidence interval of 95% in the SCA frequency in relation with the group control (p = 0.63). The percentages of chromatid and chromosome aberrations were 77.3 and 22.7%, respectively. There were no statistically significant differences found.
Conclusion: Damage genototoxic, measured through the SCA test in vitro of the Antracol WP 70 in human lymphocyte cultures, is not reported. To continue with genetic toxicology and cytogenetic studies in Boyacá Department (State) to solve doubts about the mutagenic, carcinogenic and teratogenics effects of the different physical, chemical and biological compounds in the living beings is recommended.