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  1. Home /
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  3. Vol 45 No 3 (2014) /
  4. Original Articles

Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation

  • Abstract
  • Keywords
  • Author Biographies
  • References

Abstract

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p> 0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p> 0.001). Hovewer, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

Authors

  • Miguel Alcaraz University of Murcia-Spain.
  • Samuel Quesada Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia
  • David Armero University of Murcia. 30100 Espinardo. Murcia. Spain.
  • Rocio Martín-Gil Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain.
  • Amparo Olivares University of Murcia-Spain.
  • Daniel Achel Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana.

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Keywords

  • anaesthesia
  • genotoxicity
  • micronucleus
  • radiation effects

Author Biographies


, University of Murcia-Spain.
Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.

, Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia
Radiology and Physical Medicine Department

, University of Murcia. 30100 Espinardo. Murcia. Spain.

Nursing Department. Faculty of Nursing. University of Murcia. 30100 Espinardo. Murcia. Spain.


, Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain.
Anesthesiology Department. Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain

, University of Murcia-Spain.

Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.


, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana.
Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana

References

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Alcaraz M, Acevedo C, Castillo J, Benavente-García O, Armero D, Vicente V, et al. Liposoluble antioxidants provide an effective radioprotective barrier. Br J Radiol. 2009;82:605–609.

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Published
2014-09-30
Submitted
2013-04-09
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How to Cite
Alcaraz, M., Quesada, S., Armero, D., Martín-Gil, R., Olivares, A., & Achel, D. (2014). Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation. Colombia Médica, 45(3), 104-109. https://doi.org/10.25100/cm.v45i3.1327
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Vol 45 No 3 (2014)
Section
Original Articles

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