Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation

https://doi.org/10.25100/cm.v45i3.1327
anaesthesia genotoxicity micronucleus radiation effects

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Published: Sep 30, 2014
Issue: Vol. 45 No. 3 (2014)
Section Original Articles

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Main Article Content

Authors
  • Miguel Alcaraz +
  • Samuel Quesada +
  • David Armero +
  • Rocio Martín-Gil +
  • Amparo Olivares +
  • Daniel Achel +
Abstract

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p> 0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p> 0.001). Hovewer, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

Author Biographies

Miguel Alcaraz, University of Murcia-Spain.

Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.

Samuel Quesada, Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia

Radiology and Physical Medicine Department

David Armero, University of Murcia. 30100 Espinardo. Murcia. Spain.

Nursing Department. Faculty of Nursing. University of Murcia. 30100 Espinardo. Murcia. Spain.

Rocio Martín-Gil, Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain.

Anesthesiology Department. Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain

Amparo Olivares, University of Murcia-Spain.

Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.

Daniel Achel, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana.

Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana
How to Cite
Alcaraz, M., Quesada, S., Armero, D., Martín-Gil, R., Olivares, A., & Achel, D. (2014). Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation. Colombia Médica, 45(3), 104–109. https://doi.org/10.25100/cm.v45i3.1327
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Received 2013-04-09
Accepted 2014-08-15
Published 2014-09-30

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